E. coli Monitoring in Streams by Volunteers: A Training Workshop

Session Co-Chairs: Lois Wolfson, Michigan State University, Jerry Iles, Ohio State University Extension, Barbara Liukkonen, University of Minnesota and Kristine Stepenuck, University of Wisconsin-Extension and Wisconsin DNR

Contamination of surface water by fecal matter threatens public health and is a common public health concern. While monitoring is important to ensure safe recreational opportunities, reduced state budgets and shortages of state agency personnel make regular monitoring difficult. In 2004, volunteer monitoring programs in six upper Midwestern states initiated a three year CSREES funded project to evaluate five test methods and kits for monitoring E. coli bacteria that are suitable for home use (see http://www.usawaterquality.org/volunteer/EColi/ for more information on the project). The methods were evaluated by comparing E. coli test kit results determined by volunteers to results determined through certified laboratory analyses and by assessing volunteer preferences for using the methods. After a year of study, two test methods were selected to be used in all participating states. A comprehensive training program was developed and used in all six states to assure consistency. This ensured that volunteers were provided with sufficient background information about E. coli bacteria and comparable hands-on learning opportunities to become skilled with sampling techniques and monitoring methods. The training was evaluated and modified over three years to meet participants’ needs and to achieve project goals most effectively.

This half day workshop will provide participants with this refined training to use in their own states. The training will begin with an introduction to E. coli monitoring, including its importance and relevance to water-based recreational activities. Participants will learn how, where, and when to collect water samples and how to ensure volunteer safety. Hands-on training will be provided using Coliscan Easygel and 3M Petrifilm methods. Participants will practice quantifying pre-prepared plates and learn incubation and proper disposal procedures. Recording and calculating data and sources for bacteria monitoring supplies and equipment will also be included.

Each participant will also receive a copy of the Citizens Monitoring Bacteria manual.